Overview
Mini2P – Minituarized Two-Photon Microscope
- Stable Two-Photon Imaging without Impediment to Specimen Behavior*
- Fast Multiplane Imaging with a Microtunable Lens (<0.4 ms Response Time)
- Simultaneous Measurement of Hundreds of Neurons is Possible
- On-Board Detection with a High-Sensitivity Silicon Photomultiplier (SiPM)
- Two MEMS Scanner Configurations:
- Fast Scanning: 0.17 mm2 FOV, 40 Hz (256 x 256 Pixels)
- Large FOV: 0.25 mm2 FOV, 15 Hz (256 x 256 Pixels)
The Mini2P’s lightweight, head-mounted design allows for stable calcium imaging without impediment to the sample. Interchangeable objectives facilitate use with cranial windows, prisms, and GRIN lenses for imaging tissue at different depths and orientations. The Mini2P allows for hundreds of neurons to be monitored in a single FOV, while visualization of up to a thousand cells is achievable through multiplane imaging controlled through the built-in microtunable lens.
A near-infrared, 920 nm femtosecond pulsed laser provides ideal excitation for two-photon imaging of GFP. Fiber coupling with a hollow-core photonic crystal fiber creates a stable system without complicated alignment procedures. Laser scanning is performed by a 2D microelectromechanical system (MEMS) device that is available for either fast scanning or large FOV applications. Signal detection is achieved with an on-board, dual-color silicon photomultiplier (SiPM) detector, eliminating the need for a fiber collection bundle that could impede movement. Laser scanning, signal detection, and z-scanning are all integrated into a flexible circuit controlled by a single miniaturized coax cable bundle and dedicated control box.